Western Blotting Analysis:
Representative lot data.
RAW264.7 lysate was probed with Anti-MILI (1:1,000 dilution).
Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates MILI (~109 kDa).
An uncharacterized bands at ~30, 55, and 60 kDa may be observed in some lysates.