Piwil2

Gene Symbol Piwil2
Entrez Gene 57746
Alt Symbol Piwil1l, mili
Species Mouse
Gene Type protein-coding
Description piwi-like RNA-mediated gene silencing 2
Other Description Miwi like|Miwi likw|piwi like homolog 2|piwi-like homolog 2|piwi-like protein 2
Swissprots Q8CDG1 Q99MV6 Q3TQE8 Q9JMB6 A6H617
Accessions EDL35904 Q8CDG1 AB032605 BAA93706 AF285586 AAK31965 AK030116 BAC26791 AK047771 AK163647 BAE37436 BC138444 AAI38445 BC145717 AAI45718 XM_006519328 XP_006519391 XM_006519329 XP_006519392 NM_021308 NP_067283
Function Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer- independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piR
Subcellular Location Cytoplasm {ECO:0000269|PubMed:11578866, ECO:0000269|PubMed:18922463, ECO:0000269|PubMed:19114715, ECO:0000269|PubMed:19465913, ECO:0000269|PubMed:19584108}. Note=Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
Tissue Specificity Expressed in adult testis, specifically in spermatocytes and in spermatogonia. Only detected in primordial germ cells of both sexes. Widely expressed in tumors. Also present at early stages of oocyte growth. Expressed in brain (at protein level). {ECO:0000269|PubMed:11279525, ECO:0000269|PubMed:11578866, ECO:0000269|PubMed:12906857, ECO:0000269|PubMed:18404146, ECO:0000269|PubMed:18922463, ECO:0000269|PubMed:19114715, ECO:0000269|PubMed:19377467}.
Top Pathways Dorso-ventral axis formation

Mili (D14F5) XP ® Rabbit mAb - 5940 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D14F5
Western blot analysis of extracts from mouse testis and mouse brain using Mili (D14F5) XP ® Rabbit mAb (upper) or β-Actin Antibody #4967 (lower).

Mili Antibody - 2071 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various mouse tissues using Mili Antibody (upper panel) or β-Actin Antibody (#4967) (lower panel).

PIWIL2 (G-1) - sc-377258 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PIWIL2 (G-1): sc-377258. Western blot analysis of PIWIL2 expression in rat testis tissue extract (A) and F9 whole cell lysate (B).

PIWIL2 (D-5) - sc-377347 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PIWIL2 (D-5): sc-377347. Western blot analysis of PIWIL2 expression in F9 whole cell lysate (A) and rat testis tissue extract (B).

PIWIL2 (G-16) - sc-67501 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PIWIL2 (P-15) - sc-67504 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PIWIL2 (P-15): sc-67504. Western blot analysis of PIWIL2 expression in rat testis tissue extract.

Anti-PIWIL2 antibody - ab36764 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PIWIL2 antibody (ab36764) at 1 µg/ml + Mouse Testis Whole Tissue Lysate at 20 µgSecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.

Anti-PIWIL2 antibody - ab26408 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PIWIL2 antibody (ab26408) at 1 µg/ml + Mouse Testis Whole Tissue Lysate at 20 µgSecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.

PIWIL2 Antibody - NBP2-24590 from Novus Biologicals

Prices $99.00, $229.00
Sizes 25 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: PIWIL2 Antibody [NBP2-24590] - Analysis of Mili in mouse embryo testis lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

PIWIL2 antibody - orb77200 from Biorbyt

Prices $425.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed paraffin embedded colon showing nuclear staining using PIWIL2 antibody

PIWIL2 antibody - orb77201 from Biorbyt

Prices $425.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Western blot analysis of mouse testis lysates using PIWIL2 antibody

PIWIL2 antibody - 10R-8526 from Fitzgerald

Prices $295.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 11C11

PIWIL2 Antibody - abx037940 from Abbexa

Host Rabbit
Clonality Polyclonal

PIWIL2 Antibody - abx038372 from Abbexa

Host Rabbit
Clonality Polyclonal

Mili / PiwiL2 antibody (mAb) - 61143 from ActiveMotif

Prices $95.00, $410.00
Sizes 10 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 17.8

Anti-PIWIL2 monoclonal antibody, clone 12D12 - CAB-10465MH from Creative Diagnostics

Host Mouse
Clonality Monoclonal
Clone 12D12

Anti-PIWIL2 polyclonal antibody - CABT-LM000173 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

PIWIL2 Monoclonal Antibody (11C11) - MA5-17208 from Thermo Fisher Scientific

Prices $349.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 11C11
Western blot analysis of PIWIL2 using anti-PIWIL2 monoclonal antibody (MA5-17208) on Mouse testis tissue lysate.

PIWIL2, Polyclonal Antibody - MBS852675 from MyBioSource

Prices $330.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

PIWIL2, Polyclonal Antibody - MBS852201 from MyBioSource

Prices $330.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MILI Antibody - ABE278 from EMD Millipore

Prices $279.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Mili/Piwil-2 Antibody, clone 13E-3 - MABE363 from EMD Millipore

Prices $319.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 13E-3

PIWIL2 antibody - AM50454PU-N from Acris Antibodies

Prices $390.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 13E-3
Western Blot Analysis:Representative lot data.Mouse testis tissue lysate was probed with Cat. No. AM50454PU-N, Anti-Mili/Piwil-2, clone 13E-3 (0.5 µg/mL). Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.Arrow indicates Mili/Piwil-2 (~110 kDa)

Anti-Mili/Piwil-2 Antibody, clone 13E-3 - MABE363 from Sigma-Aldrich

Prices $319.00
Sizes mabe363
Clonality Monoclonal
Western Blot Analysis:<br />Representative lot data.<br />Mouse testis tissue lysate was probed with Cat. No. MABE363, Anti-Mili/Piwil-2, clone 13E-3 (0.5 µg/mL). Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.<br />Arrow indicates Mili/Piwil-2 (~110 kDa)

Anti-MILI Antibody - ABE278 from Sigma-Aldrich

Clonality Polyclonal
Western Blotting Analysis: <br />Representative lot data. <br />RAW264.7 lysate was probed with Anti-MILI (1:1,000 dilution). <br />Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. <br />Arrow indicates MILI (~109 kDa). <br />An uncharacterized bands at ~30, 55, and 60 kDa may be observed in some lysates.
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