Anti-mouse Tks4 Antibody

Name	Anti-mouse Tks4 Antibody
Supplier	Sigma-Aldrich
Catalog	09-260
Prices	$319.00
Sizes	09-260
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human, Mouse
Purity/Format	Protein A Purfied
Description	Polyclonal
Gene	SH3PXD2B
Supplier Page	Shop

Product images

Immunocytochemistry:<br />3T3-Src (Y527F) transformed cells (A) and 3T3 cells (B) were plated and fixed then stained with a 1:3000 dilution of Anti-mouse Tks4. Proteins were visualized using an anti-rabbit secondary conjugated to FITC (See Buschman, 2009 reference for further detail).<br />

Immunocytochemistry:
3T3-Src (Y527F) transformed cells (A) and 3T3 cells (B) were plated and fixed then stained with a 1:3000 dilution of Anti-mouse Tks4. Proteins were visualized using an anti-rabbit secondary conjugated to FITC (See Buschman, 2009 reference for further detail).

Western Blot Analysis:<br />Representative lot data.<br /><br />10 µg of Anti-mouse Tks4 was used to immunoprecipitate from 500 µg of NIH3T3 whole cell lysate The antibody was collected with Protein A beads and eluted with sample buffer. 50 µg of NIH3T3 IP cell lysate was then resolved via SDS-PAGE, transferred to PVDF and probed with 3 µg/mL of the same Anti-Tks4 polyclonal antibody. Proteins were visualized using an anti-rabbit secondary antibody conjugated to HRP that does not recognize denatured IgG. Detection using a chemiluminescence system (Cat. No. 17-373).<br /><br />Arrow indicates Tks4 (~ 130 kDa).<br />

Western Blot Analysis:
Representative lot data.

10 µg of Anti-mouse Tks4 was used to immunoprecipitate from 500 µg of NIH3T3 whole cell lysate The antibody was collected with Protein A beads and eluted with sample buffer. 50 µg of NIH3T3 IP cell lysate was then resolved via SDS-PAGE, transferred to PVDF and probed with 3 µg/mL of the same Anti-Tks4 polyclonal antibody. Proteins were visualized using an anti-rabbit secondary antibody conjugated to HRP that does not recognize denatured IgG. Detection using a chemiluminescence system (Cat. No. 17-373).

Arrow indicates Tks4 (~ 130 kDa).

Product References

The SRC substrate Tks5, podosomes (invadopodia), and cancer cell invasion. - The SRC substrate Tks5, podosomes (invadopodia), and cancer cell invasion.

Courtneidge SA, Azucena EF, Pass I, Seals DF, Tesfay L. Cold Spring Harb Symp Quant Biol. 2005;70:167-71.

The novel adaptor protein Tks4 (SH3PXD2B) is required for functional podosome - The novel adaptor protein Tks4 (SH3PXD2B) is required for functional podosome

Buschman MD, Bromann PA, Cejudo-Martin P, Wen F, Pass I, Courtneidge SA. Mol Biol Cell. 2009 Mar;20(5):1302-11.