Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CUL3 mAb.
Left : Western blot analysis of immunized recombinant protein, using anti-CUL3 monoclonal antibody. Right : CBB staining of immunized recombinant protein.
Detection of CUL3 by Western blot. Samples: Whole cell lysate from human HeLa (H, 50 ug) , mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. Predicted molecular weight: 88 kDa
Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-CUL3 mAb. Predicted molecular weight: 88 kDa