CUL3

Gene Symbol CUL3
Entrez Gene 8452
Alt Symbol CUL-3, PHA2E
Species Human
Gene Type protein-coding
Description cullin 3
Other Description cullin-3
Swissprots B8ZZC3 Q9UBI8 O75415 A8K536 Q13618 Q9UET7 Q569L3
Accessions AAX93287 AAY24157 EAW70828 EAW70829 EAW70830 Q13618 AB014517 BAA31592 AF052147 AAC28621 AF062537 AAC36682 AF064087 AAC36304 AK098531 AK291151 BAF83840 AK299644 BAH13086 AK308496 AY007151 AY337761 AAQ01660 BC031844 AAH31844 BC039598 AAH39598 BC092409 AAH92409 BQ688156 U58089 AAC50546 XM_006712800 XP_006712863 XM_011511994 XP_011510296 XM_011511995 XP_011510297 XM_011511996 XP_011510298 XM_011511997 XP_011510299 NM_001257197 NP_001244126 NM_001257198 NP_001244127 NM_003590 NP_003581
Function Core component of multiple cullin-RING-based BCR (BTB- CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the substrate recognition component. BCR(KLHL42) is involved in ubiquitination of KATNA1. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, BRMS1, H2AFY and DAXX, GLI2 and GLI3. Can also form a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL; these complexes have
Subcellular Location Nucleus. Golgi apparatus.
Tissue Specificity Widely expressed.
Top Pathways Ubiquitin mediated proteolysis

CUL3 Antibody - 2759 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lysates, using CUL3 Antibody.

CUL-3 (C-18) - sc-8556 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CUL-3 (C-18): sc-8556. Western blot analysis of CUL-3 expression in NIH/3T3 whole cell lysate.

CUL-3 (C-3) - sc-166054 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CUL-3 (C-3): sc-166054. Western blot analysis of CUL-3 expression in non-transfected: sc-117752 (A) and human CUL-3 transfected: sc-111606 (B) 293T whole cell lysates.

CUL-3 (G-8) - sc-166110 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CUL-3 (G-8): sc-166110. Western blot analysis of CUL-3 expression in non-transfected: sc-117752 (A) and human CUL-3 transfected: sc-111606 (B) 293T whole cell lysates.

CUL-3 (H-1) - sc-166053 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CUL-3 (H-1): sc-166053. Western blot analysis of CUL-3 expression in non-transfected: sc-117752 (A) and human CUL-3 transfected: sc-111606 (B) 293T whole cell lysates.

CUL-3 (L-18) - sc-5322 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CUL-3 (H-293) - sc-11385 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CUL-3 (H-293): sc-11385. Western blot analysis of CUL-3 expression in non-transfected: sc-117752 (A) and human CUL-3 transfected: sc-111606 (B) 293T whole cell lysates.

CUL-3 (3) - sc-136285 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
CUL-3 (3): sc-136285. Western blot analysis of CUL-3 expression in rat cerebrum tissue extract.

Anti-Cullin 3 antibody - ab194584 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-GPR37L1 antibody (ab194568) at 1/500 dilution + Jurkat cell lysate

Anti-Cullin 3 antibody - ab118960 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab118960 at 1/500 staining Cullin 3 in Human breast tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen

Anti-Cullin 3 antibody - ab190873 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-Cullin 3 antibody (ab190873) at 1 µg/ml + Human heart lysate at 35 µgdeveloped using the ECL technique

Anti-Cullin 3 antibody [EPR3195] - ab108407 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3195
All lanes : Anti-Cullin 3 antibody [EPR3195] (ab108407) at 1/5000 dilutionLane 1 : HeLa cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : PC12 cell lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.

Anti-Cullin 3 antibody [EPR3196Y] - ab75851 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3196Y
Overlay histogram showing HeLa cells stained with ab75851 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75851, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Cullin 3 / CUL3 Antibody (aa293-554) IHC-plus™ - LS-B11690 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-Cullin 3 / CUL3 Antibody (clone 2236C1a) - LS-C342431 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 2236C1a
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CUL3 mAb.

Anti-Cullin 3 / CUL3 Antibody (N-Terminus) - LS-C6260 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Cullin 3 / CUL3 Antibody - LS-C63987 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-Cullin 3 / CUL3 Antibody (aa650-700) - LS-C63990 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Cullin 3 / CUL3 Antibody (aa1-12) - LS-C90298 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Cullin 3 / CUL3 Antibody (N-Terminus) - LS-C128431 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Cullin 3 / CUL3 Antibody (Internal, clone EPR3195) - LS-C105465 from LifeSpan Bioscience

Host Rabbit
Clonality Monoclonal
Clone EPR3195
Western blot analysis on (A) HeLa, (B) SH-SY5Y, (C) PC12 and (D) NIH/3T3 cell lysates using anti-CUL3 antibody (LS-C105465), dilution 1:5000.

Anti-Cullin 3 / CUL3 Antibody (aa1-50) - LS-C198854 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human thyroid gland tissue, using Cullin 3 Antibody. The sample on the right was incubated with synthetic peptide.

Anti-Cullin 3 / CUL3 Antibody (aa352-656) - LS-C185558 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Cullin 3 antibody detects Cullin 3 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human CUL3-transfected 293T cells. 7.5 % SDS-PAGE. Cullin 3 antibody dilution:1:5000

Anti-Cullin 3 / CUL3 Antibody (C-Terminus, clone EPR3196Y) - LS-C105449 from LifeSpan Bioscience

Host Rabbit
Clonality Monoclonal
Clone EPR3196Y
Western blot analysis on (A) HeLa, (B) SH-SY5Y, (C) PC12 and (D) 3T3 cell lysates using anti-CUL3 antibody (LS-C105449), dilution 1:20000.

Anti-Cullin 3 / CUL3 Antibody (clone 1A3) - LS-C198565 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 1A3
Detection limit for recombinant GST tagged CUL3 is 1 ng/ml as a capture antibody.
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