Western blot analysis of activated recombinant HER2/ErbB2 and HER1/EGFR tyrosine kinase proteins, using Phospho-HER2/ErbB2 (Tyr1248) (upper), EGF Receptor (D38B1) XP ® Rabbit mAb #4267 (middle) or HER2/ErbB2 (29D8) Rabbit mAb #2165 (lower). Phospho-HER2/ErbB2 (Tyr1248) specifically detects phosphorylated HER2/ErbB2 but not phosphorylated HER1/EGFR.
Western blot analysis of T47D and MCF7 cells untreated or treated with Neuregulin (100 ng/ml) for 5 minutes, using Phospho-HER2/ErbB2 (Tyr1248) Antibody (upper) or HER2/ErbB2 (D8F12) XP® Rabbit mAb #4290 (lower).