Western blot analysis of extracts from T-47D cells, untreated (-) or treated (+) with Human Neuregulin-1 (hNRG-1) #5218 (100 ng/ml, 5 min), using Phospho-HER3/ErbB3 (Tyr1328) (E1J1T) Rabbit mAb (upper) and HER3/ErbB3 (1B2E) Rabbit mAb #4754 (lower).
Immunoprecipitation of phospho-Her3/ErbB3 (Tyr1328) from T-47D cell extracts treated with Human Neuregulin-1 (hNRG-1) #5218 (100 ng/ml, 5 min) using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-Her3/ErbB3 (Tyr1328) (E1J1T) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Her3/ErbB3 (Tyr1328) (E1J1T) Rabbit mAb.