HER3/ErbB3 (D22C5) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 12708
• Prices: $109.00, $282.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D22C5
• Applications: WB IP IHC-P ICC/IF FC
• Species Reactivities: Human, Mouse, Rat
• Antigen: Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the carboxy terminus of human ErbB3 protein.
• Description: Rabbit Monoclonal
• Gene: ERBB3

Product images

Western blot analysis of extracts from MCF7 (HER3+) and MDA-MB-231 (HER3-) cells using HER3/ErbB3 (D22C5) XP ® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunohistochemical analysis of paraffin-embedded non-small cell lung carcinoma using HER3/ErbB3 (D22C5) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded breast carcinoma using HER3/ErbB3 (D22C5) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded 293T cell pellets transfected with human erbB family members HER3, EGFR, HER2 and HER4 (from left to right as indicated) using HER3/ErbB3 (D22C5) XP ® Rabbit mAb (top panels). Transfections were confirmed using EGF Receptor (D38B1) XP ® Rabbit mAb #4267 (lower left), HER2/ErbB2 (D8F12) XP ® Rabbit mAb #4290 (lower middle) and HER4/ErbB4 (111B2) Rabbit mAb #4795 (lower right).

Immunohistochemical analysis of paraffin-embedded ovarian serous adenocarcinoma using HER3/ErbB3 (D22C5) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded MCF7 (left) or MDA-MB-231 (right) cell pellets using HER3/ErbB3 (D22C5) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of MCF7 cells (HER3+, left) and MDA-MB-231 cells (HER-, right) using HER3/ErbB3 (D22C5) XP ® Rabbit mAb (green). Blue pseudocolor= DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of MCF7 cells using HER3/ErbB3 (D22C5) XP ® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900. Anti-rabbit IgG (H+L), F(ab')2 fragment (Alexa Fluor ® 488 conjugate) #4412 was used as a secondary antibody.