Western blot analysis of extracts from various cell lines using HEXIM1 (D5Y5K) Rabbit mAb
Immunoprecipitation of HEXIM1 from HeLa cell extracts, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or HEXIM1 (D5Y5K) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using HEXIM1 (D5Y5K) Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells using HEXIM1 (D5Y5K) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with JQ1 (500nM, 72 hours; green), using HEXIM1 (D5Y5K) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) was used as a secondary antibody.