Western blot analysis of FAK phosphorylation in NIH/3T3 cells treated with anisomycin. Blots were probed with FAK (C-20): sc-558 (A), p-FAK (Ser 722)-R: sc-16662-R preincubated with its cognate phosphorylated peptide (B) and p-FAK (Ser 722)-R: sc-16662-R preincubated with its cognate non-phosphorylated peptide (C).
p-FAK (Ser 722): sc-16662. Western blot analysis of FAK phosphorylation in 293T whole cell lysate.
p-FAK (Ser 722)-R: sc-16662-R. Western blot analysis of FAK phosphorylation in non-transfected 293T: sc-117752 (A), human FAK transfected 293T: sc-171032 (B) and HeLa (C) whole cell lysates.
Western blot analysis of FAK phosphorylation in non-transfected: sc-117752 (A,D), untreated human FAK transfected: sc-114600 (B,E) and lambda protein phosphatase treated human FAK transfected: sc-114600 (C,F) 293T whole cell lysates. Antibodies tested include p-FAK (Ser 722)-R: sc-16662-R (A,B,C) and FAK (C-903): sc-932 (D,E,F).
Western blot analysis of FAK phosphorylation in non-transfected: sc-117752 (A,D), untreated human FAK transfected: sc-114600 (B,E) and lambda protein phosphatase (sc-200312A) treated human FAK transfected: sc-114600 (C,F) 293T whole cell lysates. Antibodies tested include p-FAK (Ser 722)-R: sc-16662-R (A,B,C) and FAK (C-903): sc-932 (D,E,F).
p-FAK (Ser 722): sc-16662. Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic, membrane and nuclear staining of urothelial cells.