Detection of Human DHX36 by Immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-DHX36 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Detection of Human DHX36 by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug) and Jurkat (50 ug) cells. Antibodies: Affinity purified rabbit anti-DHX36 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human DHX36 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DHX36 antibody used for IP at 6 ug/mg lysate. DHX36 was also immunoprecipitated by a previous lot (-1) of this antibody. For blotting immunoprecipitated DHX36, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Mouse DHX36 by Western Blot. Samples: Whole cell lysate from NIH3T3 (50 ug), TCKM (50 ug) and Renca (50 ug) cells. Antibodies: Affinity purified rabbit anti-DHX36 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.