DIO2 Antibody IHC of formalin-fixed and paraffin-embedded mouse brain followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the mouse DIO2 Antibody for immunohistochemistry.
Western blot of DIO2 Antibody in MCF-7 cell line lysates (35 ug/lane). DIO2 (arrow) was detected using the purified antibody.
Western blot of lysates from human heart and human skeletal muscle tissue lysate (from left to right), using DIO2 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
DIO2 Antibody flow cytometry of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.