Western blot analysis of NIH/3T3 cells (A,C,E) and NIH/3T3 cells transfected with a Fyn expression vector (B,D,F) using Fyn (FYN3): sc-16 (A,B), Fyn (FYN3)-G: sc-16-G (C,D) and Fyn (15): sc-434 (E,F).
Western blot analysis of Fyn expression in Fyn-transfected NIH/3T3 (A,B) whole cell lysates. Antibodies tested include Fyn (FYN3): sc-16 (A) and Fyn (15): sc-434 (B).
Fyn (FYN3)-G: sc-16-G. Immunofluorescence staining of methanol-fixed NIH/3T3 cells transfected with Fyn showing membrane staining.
Fyn (FYN3) PE: sc-16 PE. Intracellular FCM analysis of fixed and permeabilized (A) H4 cells and (B) human peripheral blood lymphocytes, showing specificity for the brain isoform of Fyn. Black line histograms represents the isotype control, normal rabbit IgG: sc-3871.
Fyn (FYN3) PE: sc-16 PE. Intracellular FCM analysis of human peripheral blood lymphocytes showing specificity for the brain isoform of Fyn. Solid black line histograms represent control rabbit IgG.
Fyn (FYN3) PE: sc-16 PE. Intracellular FCM analysis of fixed and permeabilized (A) H4 cells and (B) human peripheral blood lymphocytes, showing specificity for the brain isoform of Fyn. Black line histograms represents the isotype control, normal rabbit IgG: sc-3871.
Fyn (FYN3): sc-16. Western blot analysis of Fyn expression in non-transfected 293T: sc-117752 (A), human Fyn transfected 293T: sc-115186 (B) and HeLa (C) whole cell lysates.
Fyn (FYN3)-G: sc-16-G. Western blot analysis of Fyn expression in non-transfected: sc-117752 (A) and human Fyn transfected: sc-115186 (B) 293T whole cell lysates.
Fyn (FYN3): sc-16. Western blot analysis of Fyn expression in non-transfected 293: sc-110760 (A), human Fyn transfected 293: sc-158527 (B) and NIH/3T3 (C) whole cell lysates.
Fyn (FYN3): sc-16-G. Western blot analysis of Fyn expression in non-transfected: sc-110760 (A) and human Fyn transfected: sc-158527 (B) 293 whole cell lysates.