Western blot analysis of control (A,C,E,G) and UV-treated (B,D,F,H) JNK1 (A,B,E,F) and JNK2 (C,D,G,H) transfected COS cells. Antibodies tested include p-JNK (G-7): sc-6254 (A-D) and JNK1 (FL): sc-571 (E-H).
JNK (FL) PE: sc-571 PE. Intracellular FCM analysis of fixed and permeabilized A431 cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-3871.
JNK1 (FL): sc-571. Western blot analysis of JNK1 and JNK2 expression in K-562 (A), A-431 (B), NIH/3T3 (C), HeLa (D), RAW 264.7 (E) and Jurkat (F) whole cell lysates.
JNK (FL): sc-571. Western blot analysis of control (A,C) and UV-treated (B,D) JNK p46 (A,B) and JNK p54 (C,D) transfected COS cells.
JNK (FL): sc-571. Western blot analysis of JNK2 expression in non-transfected 293T: sc-117752 (A), human JNK2 transfected 293T: sc-115269 (B) and HeLa (C) whole cell lysates.
JNK (FL): sc-571. Western blot analysis of JNK3 expression in non-transfected: sc-117752 (A) and mouse JNK3 transfected: sc-121159 (B) 293T whole cell lysates and mouse brain tissue extract (C).
Western blot analysis of JNK phosphorylation in untreated (A,D), anisomycin treated (B,E) and anisomycin and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-JNK (Thr 183/Tyr 185)-R: sc-12882-R (A,B,C) and JNK (FL): sc-571 (D,E,F).
Western blot analysis of JNK phosphorylation in untreated (A,D), anisomycin treated (B,E) and anisomycin and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-JNK (G-7): sc-6254 (A,B,C) and JNK (FL): sc-571 (D,E,F).
Western blot analysis of JNK phosphorylation in untreated (A,D), anisomycin treated (B,E) and anisomycin and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-JNK (14.Thr 183/Tyr 185): sc-293136 (A,B,C) and JNK (FL): sc-571 (D,E,F).
Western blot analysis of JNK phosphorylation in untreated (A,D), anisomycin treated (B,E) and anisomycin and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-JNK (29.Thr 183/Tyr 185): sc-293137 (A,B,C) and JNK (FL): sc-571 (D,E,F).
Western blot analysis of JNK phosphorylation in untreated (A,D), anisomycin treated (B,E) and anisomycin and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-JNK (89.Thr 183/Tyr 185): sc-293138 (A,B,C) and JNK (FL): sc-571 (D,E,F).