MAPK8

Gene Symbol MAPK8
Entrez Gene 5599
Alt Symbol JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c
Species Human
Gene Type protein-coding
Description mitogen-activated protein kinase 8
Other Description JUN N-terminal kinase|MAP kinase 8|c-Jun N-terminal kinase 1|mitogen-activated protein kinase 8 isoform JNK1 alpha1|mitogen-activated protein kinase 8 isoform JNK1 beta2|stress-activated protein kinase 1|stress-activated protein kinase 1c
Swissprots P45983 Q308M2 D3DX92 Q15712 B5BTZ5 B7ZLV4 D3DX88 Q15709 Q15713
Accessions EAW93129 EAW93130 EAW93131 EAW93132 EAW93133 EAW93134 EAW93135 EAW93136 EAW93137 EAW93138 P45983 AA808177 AB074280 AB451231 BAG70045 AB451271 BAG70085 AB451354 BAG70168 AB451398 BAG70212 AK094000 AK125150 AK292523 BAF85212 AL137667 BC130570 AAI30571 BC130572 AAI30573 BC144063 AAI44064 DA159335 DQ234352 ABB29981 HQ258413 ADR83167 L26318 AAA36131 U34822 AAC50607 U35004 AAC50610 U35005 AAC50611 XM_011539953 XP_011538255 XM_011539954 XP_011538256 XM_011539955 XP_011538257 XM_011539956 XP_011538258 XM_011539957 XP_011538259 XM_011539958 XP_011538260 NM_001278547 NP_001265476 NM_001278548 NP_001265477 NM_002750 NP_002741 NM_139046 NP_620634 NM_139049 NP_620637
Function Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells
Subcellular Location Cytoplasm {ECO:0000269|PubMed:21148294}. Nucleus {ECO:0000269|PubMed:21148294}.
Top Pathways Inflammatory mediator regulation of TRP channels, Sphingolipid signaling pathway, Herpes simplex infection, Dopaminergic synapse, Choline metabolism in cancer

Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate) - 5755 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Mouse
Clonality Monoclonal
Clone G9
Flow cytometric analysis of THP-1 cells, untreated (blue) or anisomycin-treated (green), using Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate).

Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Biotinylated) - 5136 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone G9
Western blot analysis of extracts from HT-29 cells, untreated or UV-treated, using Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Biotinylated) and detected with Streptavidin-HRP #3999.

Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Alexa Fluor ® 647 Conjugate) - 9257 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Mouse
Clonality Monoclonal
Clone G9
Flow cytometric analysis of THP1 cells, untreated (blue) or anisomycin-treated (green), using Phospho-SAPK/JNK (T183/Y185) (G9) Mouse mAb (Alexa Fluor ® 647 Conjugate) (#9257) compared to a nonspecific control antibody (red).

Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb - 4668 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 81E11
Western blot analysis of extracts from 293 cells, untreated or UV-treated, NIH/3T3 cells, untreated or UV-treated and C6 cells, untreated or anisomycin-treated, using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb.

Phospho-SAPK/JNK (Thr183/Tyr185) Antibody - 9251 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 200 µl (20 western blots), 600 µl (60 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 or SK-N-MC cells treated with UV (40 mJ/cm2), using Phospho-SAPK/JNK (Thr183/Tyr185) Antibody (upper) or SAPK/JNK Antibody (lower).

Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb - 9255 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 200 µl (40 western blots), 600 µl (120 western blots)
Host Mouse
Clonality Monoclonal
Clone G9
Western blot analysis of extracts from 293 cells, untreated or UV-treated (lanes 1 and 2), NIH/3T3 cells, untreated or UV-treated (lanes 3 and 4) and C6 cells, untreated or anisomycin-treated (lanes 5 and 6), using Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb.

PhosphoPlus® SAPK/JNK (Thr183/Tyr185) Antibody Kit - 9250 from Cell Signaling Technology

Prices $489.00
Sizes 1 kit (20 western blots)
Clonality Polyclonal

Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb - 4671 from Cell Signaling Technology

Prices $287.00
Sizes 200 µl (20 western blots)
Host Rabbit
Clonality Monoclonal
Clone 98F2
Western blot analysis of purified MAPK phospho-proteins or extracts from NIH/3T3 cells treated with UV light and PDGF, using Phospho-p44/42 MAPK (Thr202/Tyr204) (197G2) Rabbit mAb (upper), Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (middle), and Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671 (lower).

JNK1 (2C6) Mouse mAb - 3708 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 2C6
Western blot analysis of HeLa cell extracts, untransfected (lane 1), mock-transfected (lane 2) or transfected with SignalSilence ® SAPK/JNK siRNA I #6232 (lane 3) or SignalSilence ® SAPK/JNK siRNA II #6233 (lane 4) for 72 hours, using JNK1 (2C6) Mouse mAb.

SAPK/JNK Antibody - 9252 from Cell Signaling Technology

Prices $99.00, $246.00, $596.00
Sizes 20 µl (2 western blots), 200 µl (20 western blots), 600 µl (60 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 and SK-N-MC cells, untreated or UV-treated (40 J/m2), using Phospho-SAPK/JNK Antibody #9251 (upper) or SAPK/JNK Antibody (lower).

JNK2 (56G8) Rabbit mAb - 9258 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 200 µl (20 western blots)
Host Rabbit
Clonality Monoclonal
Clone 56G8
Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using JNK2 (56G8) Rabbit mAb.

Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb - 5135 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone G9

JNK (D-6) - sc-137018 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
JNK (D-6): sc-137018. Western blot analysis of JNK expression in RAW 264.7 whole cell lysate.

JNK (D-9) - sc-137019 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
JNK (D-9): sc-137019. Western blot analysis of human recombinant JNK1.

JNK (E-5) - sc-137020 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
JNK (E-5): sc-137020. Western blot analysis of human recombinant JNK1.

JNK1 (G-4) - sc-398989 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
JNK1 (G-4): sc-398989. Western blot analysis of JNK1 expression in HeLa (A), A-431 (B), SK-N-MC (C), Jurkat (D) and PC-12 (E) whole cell lysates.

JNK (FL) - sc-571 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of control (A,C,E,G) and UV-treated (B,D,F,H) JNK1 (A,B,E,F) and JNK2 (C,D,G,H) transfected COS cells. Antibodies tested include p-JNK (G-7): sc-6254 (A-D) and JNK1 (FL): sc-571 (E-H).

JNK1 (F-3) - sc-1648 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of JNK1 and JNK2 expression in COS cells transfected with JNK1 (A,B,E,F,I,J) or JNK2 (C,D,G,H,K,L). Antibodies tested include JNK1 (F-3): sc-1648 (A-D), JNK1 (C-17)-G: sc-474-G (E-H) and JNK2 (FL): sc-572 (I-L).

JNK1 (G-13) - sc-46006 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
JNK1 (G-13): sc-46006. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing cytoplasmic and nuclear staining of neuronal cells.

JNK1 (37) - sc-136205 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
JNK1 (37): sc-136205. Western blot analysis of JNK1 expression in PC-12 whole cell lysate.

JNK1 (88.20) - sc-136533 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

JNK1/3 (C-17) - sc-474 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
JNK1/3 (C-17): sc-474. Western blot analysis of JNK p46 expression in K-562 (A), A-431 (B), NIH/3T3 (C), KNRK (D) and HeLa (E) whole cell lysates.

JNK1/3 (Y-12) - sc-46009 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
JNK1/3 (Y-12): sc-46009. Western blot analysis of human recombinant JNK fusion protein (A) and JNK1/3 expression in A-431 (B), KNRK (C), NIH/3T3 (D), HeLa (E), K-562 (F), 293T (G) and RAW 264.7 (H) whole cell lysates.

JNK1/3 (D-12) - sc-46007 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
JNK1/3 (D-12): sc-46007. Western blot analysis of JNK1/3 expression in RAW 264.7 whole cell lysate.

JNK1/3 (L-12) - sc-46008 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
JNK1/3 (L-12): sc-46008. Western blot analysis of JNK3 expression in non-transfected: sc-117752 (A) and mouse JNK3 transfected: sc-121159 (B) 293T whole cell lysates.
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