Western blot analysis of JNK1 and JNK2 expression in COS cells transfected with JNK1 (A,B,E,F,I,J) or JNK2 (C,D,G,H,K,L). Antibodies tested include JNK1 (F-3): sc-1648 (A-D), JNK1 (C-17)-G: sc-474-G (E-H) and JNK2 (FL): sc-572 (I-L).
JNK1 (F-3): sc-1648. Western blot analysis of JNK1 p46 expression in HeLa (A), RAW 264.7 (B), A-431 (C) and Jurkat (D) whole cell lysates.
JNK1 (F-3): sc-1648. Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
JNK1 (F-3) Alexa Fluor 488: sc-1648 AF488. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
JNK1 (F-3) Alexa Fluor 488: sc-1648 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-3890.
JNK1 (F-3) AF647: sc-1648 AF647. Intracellular FCM analysis of fixed and permeabilized Hela cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-24636.
JNK1 (F-3): sc-1648. Western blot analysis of human recombinant JNK1 fusion protein.
JNK1 (F-3): sc-1648. Western blot analysis of JNK1 expression in PC-12 (A) and NIH/3T3 (B) whole cell lysates.
JNK1 HDR Plasmid (h): sc-400048-HDR. Western blot analysis of JNK1 expression in non-transfected control(A) and puromycin, JNK1 CRISPR/Cas9 KO Plasmid: sc-400048 plus JNK1 HDR Plasmid co-transfected (B) HEK 293T whole cell lysates. Blot probed with JNK1 (F-3): sc-1648. β-Actin (C4): sc-47778 used as specificity and loading control.