Human Lung, Respiratory Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection of Human and Mouse EDC3 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3cells. Antibodies: Affinity purified rabbit anti-EDC3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of Human EDC3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-EDC3 antibody used for IP at 6 ug/mg lysate. EDC3 was also immunoprecipitated by rabbit anti-EDC3 antibody BL13994. For blotting immunoprecipitated EDC3, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.