Detection of Human EDF-1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-EDF-1 antibody used for IP at 6 ug/mg lysate. EDF-1 was also immunoprecipitated by rabbit anti-EDF-1 antibody BL14527. For blotting immunoprecipitated EDF-1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of Human EDF-1 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-EDF-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.