EDF1

Gene Symbol EDF1
Entrez Gene 8721
Alt Symbol EDF-1, MBF1
Species Human
Gene Type protein-coding
Description endothelial differentiation-related factor 1
Other Description multiprotein bridging factor 1
Swissprots Q5T5T2 O60869 Q9UIM1
Accessions CAJ42711 CBX85963 CBX85993 EAW88296 EAW88297 EAW88298 O60869 AA325420 AB002282 BAA88073 AB002283 BAA88074 BC015500 AAH15500 BI833652 BM800379 BQ279031 BT009863 AAP88865 CR541914 CAG46712 DQ891577 ABM82503 DQ894770 ABM85696 HY029111 HY086994 HY170122 NM_001281297 NP_001268226 NM_001281298 NP_001268227 NM_001281299 NP_001268228 NM_003792 NP_003783 NM_153200 NP_694880
Function Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. {ECO:0000269|PubMed:10567391, ECO:0000269|PubMed:12040021, ECO:0000269|PubMed:15112053, ECO:0000269|PubMed:9813014}.
Subcellular Location Cytoplasm. Nucleus. Note=Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin.
Tissue Specificity Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues. {ECO:0000269|PubMed:10567391, ECO:0000269|PubMed:12040021, ECO:0000269|PubMed:9813014}.

EDF1 (C-19) - sc-70259 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

EDF1 (D-18) - sc-70260 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

EDF1 (U-21) - sc-133536 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
EDF1 (U-21): sc-133536. Western blot analysis of EDF1 expression in Jurkat whole cell lysate.

Anti-EDF1 antibody - ab88316 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-EDF1 antibody (ab88316) at 5 µg/ml + immunogen (tagged, recombinant full length EDF1 protein) at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Anti-EDF1 antibody - ab118583 from Abcam

Prices $407.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab118583, at 7.5 µg/ml, staining EDF1 in formalin-fixed, paraffin-embedded Human Brain, Cortex tissue by Immunohistochemistry.

Anti-EDF1 / MBF1 Antibody (aa98-148) - LS-C290686 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human EDF-1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-EDF-1 antibody used for IP at 6 ug/mg lysate. EDF-1 was also immunoprecipitated by rabbit anti-EDF-1 antibody BL14527. For blotting immunoprecipitated EDF-1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Anti-EDF1 / MBF1 Antibody (aa122-155) - LS-C344710 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysate from zebra fish brain tissue with (DANRE)edf1 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Anti-EDF1 / MBF1 Antibody (C-Terminus) IHC-plus™ - LS-B6613 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-EDF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6613 concentration 5 ug/ml.

Anti-EDF1 / MBF1 Antibody (aa49-63) IHC-plus™ - LS-B10582 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-EDF1 / MBF1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10582 concentration 5 ug/ml.

Anti-EDF1 / MBF1 Antibody (N-Terminus) - LS-C31515 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Jurkat Cell Lysate

Anti-EDF1 / MBF1 Antibody (N-Terminus) - LS-C110065 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EDF1 antibody LS-C110065 Western blot of THP-1 cell lysate.

Anti-EDF1 / MBF1 Antibody (clone 3E6) - LS-C133988 from LifeSpan Bioscience

Prices $315.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3E6
EDF1 monoclonal antibody (M03), clone 3E6. Western Blot analysis of EDF1 expression in Hela NE.

Anti-EDF1 / MBF1 Antibody (aa9-58) - LS-C205696 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EDF1 / MBF1 antibody Western Blot of Uterus Tumor. Antibody dilution: 1 ug/ml.

Anti-EDF1 / MBF1 Antibody - LS-C335224 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of Jurkat cell lines, using EDF1 antibody.

Anti-EDF1 / MBF1 Antibody (aa2-14) IHC-plus™ - LS-B5104 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-EDF1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5104 concentration 3.75 ug/ml.

EDF1 Antibody - NBP1-84011 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: EDF1 Antibody [NBP1-84011] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

EDF1 Antibody - H00008721-B02 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: EDF1 Antibody [H00008721-B02] - analysis of EDF1 expression in transfected 293T cell line by EDF1 MaxPab polyclonal antibody (B02). Lane 1: EDF1 transfected lysate (15.98 KDa). Lane 2: Non-transfected lysate.

EDF1 Antibody (3E6.) - H00008721-M03 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E6.
Western Blot: EDF1 Antibody (3E6) [H00008721-M03] - Analysis of EDF1 expression in Hela S3 NE (Cat # L013V3).

EDF1 Antibody - NBP2-22327 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: EDF1 Antibody [NBP2-22327] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22327 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

EDF1 Antibody - H00008721-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: EDF1 Antibody [H00008721-B01] - analysis of EDF1 expression in transfected 293T cell line by EDF1 MaxPab polyclonal antibody (B01). Lane 1: EDF1 transfected lysate (16.39 KDa). Lane 2: Non-transfected lysate.

Monoclonal Anti-EDF1 antibody produced in mouse - SAB1402430 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E6
<B>Immunohistochemistry</B><BR/>Monoclonal Anti-EDF1, antibody produced in mouse, clone 3E6, purified immunoglobulin: <B>Cat. No. SAB1402430</B>: Antibody concentration: 3 μg/mL using human pancreas tissue.

Anti-EDF1, (N-terminal) antibody produced in rabbit - SAB2104652 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-EDF1, antibody produced in rabbit: <B>Cat. No. SAB2104652</B>: Immunoblotting analysis of EDF1 in THP-1 cell lysate with EDF1 anitbody at 1 μg/mL.

Anti-EDF1 antibody produced in rabbit - HPA035642 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-EDF1: <B>Cat. No. HPA035642</B>: Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.

Anti-EDF1 antibody produced in rabbit - AV37729 from Sigma-Aldrich

Prices $267.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: Jurkat (<B>Cat. No. AV37729</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1.25 μg/mL in cell type Jurkat

Anti-EDF1 antibody produced in rabbit - SAB2100648 from Sigma-Aldrich

Prices $347.50
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-EDF1: <B>Cat. No. SAB2100648</B>: Immunohistochemistry of EDF1 in Prostate tissue with EDF1 antibody at 4-8 μg/mL.
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