Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2.Anti-pan ASIC (extracellular) antibody (#AG1595), (1:500).3 -4. Anti-pan ASIC (extracellular) antibody, preincubated with the control peptide antigen.
Western blot analysis of mouse brain membranes (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 4):1-2.Anti-pan ASIC (extracellular) antibody (#AG1595), (1:500).3 -4. Anti-pan ASIC (extracellular) antibody, preincubated with the control peptide antigen.
Expression of ASIC channels in rat locus coeruleus Immunohistochemical staining of perfusion-fixed rat brain frozen sections using Anti-pan ASIC (extracellular) antibody (#AG1595), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. ASIC channel staining (green) appears in neuronal soma (arrows). Nuclei are stained with DAPI (blue).
Expression of ASIC in rat PC12 cells Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-pan ASIC (extracellular) antibody (#AG1595), (1:25), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Merge of A and B.