Published customer image:Rabbit anti ATG5-ATG12 Complex antibody used for the evaluation of ATG5-ATG12 conjugate expression in OVCAR-3 cells following induction of autophagy by western blotting.Image caption:The time course of autophagy and apoptosis induction by 30 µM resveratrol treatment. A. Live cell imaging by confocal microscopy (with 10X objective) with Cyto-ID dye in OVCAR-3 cells treated with 30 µM resveratrol at 0, 12, 24 and 48 hour time points. B. Live cell imaging by confocal microscopy (with 10X objective) with caspase 3 green detection dye in OVCAR-3 cells at 0, 12, 24 and 48 hour time points. C. OVCAR-3 cells were treated with 30 µM of resveratrol for 12 h, 24 h, and 48 h. Western blotting of total cell lysates were performed with anti-ATG5-ATG12, LC3, and caspase 3 antibodies. No cleavage of ATG5 was observed. The results shown represent an experiment, in which each time-point of treatment was duplicated.From: Lang F, Qin Z, Li F, Zhang H, Fang Z, Hao E Apoptotic Cell Death Induced by Resveratrol Is Partially Mediated by the Autophagy Pathway in Human Ovarian Cancer Cells. PLoS ONE 10: e0129196.