Atg12

Gene Symbol Atg12
Entrez Gene 67526
Alt Symbol 4931423H11Rik, A330058M13Rik, Apg12l, Atg12l
Species Mouse
Gene Type protein-coding
Description autophagy related 12
Other Description autophagy-related 12|autophagy-related protein 12|ubiquitin-like protein ATG12
Swissprots Q9CQY1 Q3TKE5 Q9D7Y5
Accessions EDL09966 EDL09967 Q9CQY1 AB066216 BAB62092 AK005405 BAB24005 AK008698 BAB25839 AK016474 BAB30256 AK039278 AK039546 AK080409 AK088255 AK167027 BAE39200 AK177781 AK177910 AK196572 AK198454 AK206569 AK208391 AK214439 AK218629 AV295996 BB853539 BC070470 AAH70470 XM_006526187 XP_006526250 NM_026217 NP_080493
Function Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus. {ECO:0000269|PubMed:11266458, ECO:0000269|PubMed:12890687, ECO:0000269|PubMed:20723759}.
Subcellular Location Cytoplasm {ECO:0000250}. Preautophagosomal structure membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane. {ECO:0000250}.
Tissue Specificity Ubiquitous.
Top Pathways RIG-I-like receptor signaling pathway, Regulation of autophagy

Atg12 Antibody (Mouse Specific) - 2011 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A20, NIH/3T3, and C2C12 cell lines using Atg12 Antibody (Mouse Specific).

Anti-Apg12 antibody - ab64208 from Abcam

Host Sheep
Clonality Polyclonal

Anti-Apg12 antibody - ab74865 from Abcam

Host Sheep
Clonality Polyclonal

ATG12 Antibody - NBP1-45349 from Novus Biologicals

Prices $245.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: ATG12 Antibody [NBP1-45349] - Analysis of ATG12 in 3T3 cell lysate (Lane 1) and rat kidney tissue lysate (Lane 2).

ATG12 / APG12 antibody - PAB8542 from Acris Antibodies

Prices $430.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of Atg12 expression in NIH/3T3 cell lysate ( Lane 1 ) and rat kidney tissue lysate ( Lane 2 ) .

Rabbit Anti-APG12/ATG12 Antibody - 168-10699 from RayBiotech

Host Rabbit

ATG12 Polyclonal Antibody - PA5-17218 from Thermo Fisher Scientific

Prices $275.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of Atg12 in NIH/3T3 cells, chloroquine-treated, using an Atg12 polyclonal antibody (Product # PA5-17218) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

ATG5-ATG12 Complex Antibody - AAM79 from AbD Serotec

Prices $350.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

ATG12 Antibody - CSB-PA002283GA01HU from Cusabio

Host Rabbit

APG12/ATG12 Antibody - 3912-100 from Biovision

Prices $245.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

RABBIT ANTI ATG5-ATG12 COMPLEX (C-TERMINAL) Antibody (0.1 mg) - OASA11600 from Aviva Systems Biology

Prices $535.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

APG12/ATG12, Polyclonal Antibody - MBS841012 from MyBioSource

Prices $150.00, $290.00
Sizes 30 µg, 100 µg
Host Rabbit
Clonality Polyclonal

anti-ATG12 antibody - ARG54815 from Arigo Biolaboratories

ARG54815 anti-ATG12 antibody WB image Western blot: 35 μg of MCF-7 cell line lysates stained with ARG54815 anti-ATG12 antibody.

APG12ATG12-Antibody - ABV10706 from Abgent, a WuXi AppTec company

Prices $89.00, $245.00
Sizes 30 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of Apg12 in 3T3 cell lysate (Lane 1) and rat kidney tissue lysate (Lane 2).

Anti-ATG12-N-Terminal-Antibody- - ABD13348 from Abgent, a WuXi AppTec company

Prices $189.00, $357.00
Sizes 50 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of human brain lysate probed with Rabbit anti Human ATG12 at 0.5(A) and 1(B) ug/ml

Anti-ATG5-ATG12-Complex-C-Terminal-Antibody- - ABD10857 from Abgent, a WuXi AppTec company

Prices $416.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Published customer image:Rabbit anti ATG5-ATG12 Complex antibody used for the evaluation of ATG5-ATG12 conjugate expression in OVCAR-3 cells following induction of autophagy by western blotting.Image caption:The time course of autophagy and apoptosis induction by 30 µM resveratrol treatment. A. Live cell imaging by confocal microscopy (with 10X objective) with Cyto-ID dye in OVCAR-3 cells treated with 30 µM resveratrol at 0, 12, 24 and 48 hour time points. B. Live cell imaging by confocal microscopy (with 10X objective) with caspase 3 green detection dye in OVCAR-3 cells at 0, 12, 24 and 48 hour time points. C. OVCAR-3 cells were treated with 30 µM of resveratrol for 12 h, 24 h, and 48 h. Western blotting of total cell lysates were performed with anti-ATG5-ATG12, LC3, and caspase 3 antibodies. No cleavage of ATG5 was observed. The results shown represent an experiment, in which each time-point of treatment was duplicated.From: Lang F, Qin Z, Li F, Zhang H, Fang Z, Hao E Apoptotic Cell Death Induced by Resveratrol Is Partially Mediated by the Autophagy Pathway in Human Ovarian Cancer Cells. PLoS ONE 10: e0129196.
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