Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3633 concentration 10 ug/ml.
Anti-ENO1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3633 concentration 10 ug/ml.
Fluorescent confocal image of C2C12 cell stained with ENO1 Antibody. C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENO1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ENO1 immunoreactivity is localized to Cytoplasm significantly.
ENO1 Antibody western blot of Jurkat,MCF-7,U251,mouse NIH/3T3 cell line and mouse brain tissue lysates (35 ug/lane). The ENO1 antibody detected the ENO1 protein (arrow).
ENO1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.