Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of hEPHA2-T45 in MCF-7 cell line lysates (35 ug/lane). EPHA2 (arrow) was detected using the purified Pab
Western blot of EPHA2 (arrow) using rabbit polyclonal hEPHA2-T45. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA2 gene (Lane 2)
Western blot of hEPHA2-T45 in mouse NIH-3T3 cell line lysates (35 ug/lane). EPHA2 (arrow) was detected using the purified antibody.
Flow cytometric of NCI-H292 cells using EphA2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.