Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 Monoclonal Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of lysate from U-251 MG cell line, using EphA5 Antibody. Antibody was diluted at 1:1000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of anti-EPHA5 Monoclonal Antibody by EPHA5 recombinant protein (Fragment). EPHA5 (Fragment) protein (arrow) was detected using the purified antibody.(1:2000)