Western blot analysis of extracts from various cell lines using HMGA1 (D6A4) XP ® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of HMGA1 from COS-7 cell extracts, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 3) or HMGA1 (D6A4) XP ® Rabbit mAb (lane 2). Lane 1 is 10% input. Western blot analysis was performed using HMGA1 (D6A4) XP ® Rabbit mAb.
Confocal immunofluorescent analysis of NCCIT (high expression; left) or MCF7 (low expression; right) cells, using HMGA1 (D6A4) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).