Western blot analysis of p-p70 S6 kinase α activation in whole cell lysates from serum-starved (A,C) and serum-starved then serum-treated (B,D) NIH/3T3 cells. Antibodies tested include p-p70 S6 kinase α (H-9): sc-8418 (A,B) and p-p70 S6 kinase α (A-6): sc-8416 (C,D).
p-p70 S6 kinase α (A-6): sc-8416. Western blot analysis of p70 S6 kinase α phosphorylation in non-transfected 293T: sc-117752 (A), mouse p70 S6 kinase α transfected 293T: sc-125770 (B) and NIH/3T3 (C) whole cell lysates.
Western blot analysis of p70 S6 kinase α phosphorylation in untreated (A,D), insulin treated (B,E) and insulin and lambda protein phosphatase (sc-200312A) treated (C,F) 293T whole cell lysates. Antibodies tested include p-p70 S6 kinase α (A-6): sc-8416 (A,B,C) and p70 S6 kinase α (C-18): sc-230 (D,E,F).
Western blot analysis of p70 S6 kinase α phosphorylation in untreated (A,D), insulin treated (B,E) and insulin treated and lambda protein phosphatase (sc-200312A) treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-p70 S6 kinase α (A-6): sc-8416 (A,B,C) and p70 S6 kinase α (C-18): sc-230 (D,E,F).
Western blot analysis of p70 S6 kinase α phosphorylation in untreated (A, D), insulin treated (B, E) and insulin and lambda protein phosphatase treated (C, F) 293T whole cell lysates. Antibodies tested include p-p70 S6 kinase α (A-6): sc-8416 (A, B, C) and p70 S6 kinase α (C-18): sc-230 (D, E, F).
p-p70 S6 kinase α (A-6): sc-8416. Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear staining of squamous epithelial cells.
Western blot analysis of p70 S6 kinase α phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) MCF7 whole cell lysates. Antibodies tested include p-p70 S6 kinase α (A-6): sc-8416 (A,B) and p70 S6 kinase α (C-18): sc-230 (C,D).
p-p70 S6 kinase α (A-6): sc-8416. Immunofluorescence staining of anisomycin-treated, methanol-fixed NIH/3T3 cells, showing cytoplasmic and nuclear localization of activated p70 S6 kinase α.
p-p70 S6 kinase α (A-6): sc-8416. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma showing nuclear localization of activated p-p70 S6 kinase α.