Western blot analysis of whole cell extracts from HeLa cells, untreated (lanes 1 and 4), treated for 1 h with Forskolin (30 μM) and IBMX (0.5 mM) (lanes 2 and 3), or treated for 16 h with paclitaxel (500 nM) (lanes 5 and 6), using Phospho-HP1γ (Ser83) Antibody (upper panel) or HP1γ Antibody #2619 (lower panel).
Confocal immunofluorescent analysis of HeLa cells, untreated (left), forskolin- and IBMX-treated (center) or λ phosphatase-treated (right), using Phospho-HP1γ (Ser83) Antibody (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red).