CBX3

Gene Symbol	CBX3
Entrez Gene	11335
Alt Symbol	HECH, HP1-GAMMA, HP1Hs-gamma
Species	Human
Gene Type	protein-coding
Description	chromobox homolog 3
Other Description	HP1 gamma homolog\|chromobox homolog 3 (HP1 gamma homolog, Drosophila)\|chromobox protein homolog 3\|heterochromatin protein 1 homolog gamma\|heterochromatin protein HP1 gamma\|heterochromatin-like protein 1\|modifier 2 protein
Swissprots	Q9P0Z6 Q9BVS3 Q99409 Q96CD7 Q13185
Accessions	EAL24235 EAW93842 EAW93843 EAW93844 EAW93845 EAW93846 Q13185 AB030905 BAA83340 AF086270 AF136630 AAF62370 AL542267 BC000954 AAH00954 BC014380 AAH14380 BX647444 BX648807 CD514780 CV575477 DB565723 EU831928 ACE87369 EU832023 ACE86685 U26312 AAB48101 XM_005249611 XP_005249668 XM_005249612 XP_005249669 NM_007276 NP_009207 NM_016587 NP_057671
Function	Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the mehtyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1.
Subcellular Location	Nucleus {ECO:0000305}. Note=Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis (Potential). {ECO:0000305}.

Phospho-HP1γ (Ser83) Antibody - 2600 from Cell Signaling Technology

Prices	$287.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of whole cell extracts from HeLa cells, untreated (lanes 1 and 4), treated for 1 h with Forskolin (30 μM) and IBMX (0.5 mM) (lanes 2 and 3), or treated for 16 h with paclitaxel (500 nM) (lanes 5 and 6), using Phospho-HP1γ (Ser83) Antibody (upper panel) or HP1γ Antibody #2619 (lower panel).

HP1γ Antibody - 2619 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of lysates from HeLa, NIH/3T3, C6 and COS cells, using HP1gamma antibody.

HP1γ (E-7) - sc-365085 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

HP1γ (E-7): sc-365085. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

HP1γ (F-1) - sc-398562 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

HP1γ (F-1): sc-398562. Western blot analysis of HP1γ expression in HeLa (A) and K-562 (B) nuclear extracts and K-562 (C), HeLa (D) and MCF7 (E) whole cell lysates.

HP1γ (N-15) - sc-10213 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

HP1γ siRNA (h): sc-35589. Western blot analysis of HP1γ expression in non-transfected control (A) and HP1γ siRNA transfected (B) HeLa cells. Blot probed with HP1γ (N-15): sc-10213. GAPDH (FL-335): sc-25778 used as specificity and loading control.

HP1γ (E-16) - sc-10214 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

HP1γ(E-16): sc-10214. Western blot analysis of HP1γ expression in NIH/3T3 nuclear extract.

Anti-HP1 gamma (unmodified S93) antibody - ab79304 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-HP1 gamma (unmodified S93) antibody (ab79304) at 1/500 dilutionLane 1 : Extracts from 293 cells with no immunizing peptideLane 2 : Extracts from 293 cells with immunizing peptide at 10 µgLysates/proteins at 30 µg per lane.Observed band size : 21 kDa (why is the actual band size different from the predicted?)

Anti-HP1 gamma antibody - ab56978 from Abcam

Prices	$385.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

HP1 gamma antibody (ab56978) used in immunofluorescence at 10ug/ml on HeLa cells.

Anti-HP1 gamma antibody - ab135119 from Abcam

Host	Rabbit
Clonality	Polyclonal

Anti-HP1 gamma antibody (ab135119) at 1/1000 dilution + HeLa total cell extract

Anti-HP1 gamma antibody [EPR10465(B)] - ab154871 from Abcam

Prices	$387.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR10465(B)

Overlay histogram showing HeLa cells stained with ab154871 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab154871, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-HP1 gamma antibody [EPR10466(B)] - ab154195 from Abcam

Prices	$379.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR10466(B)

All lanes : Anti-HP1 gamma antibody [EPR10466(B)] (ab154195) at 1/1000 dilutionLane 1 : HepG2 cell lysate Lane 2 : MOLT4 cell lysate Lane 3 : HeLa cell lysate Lysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-HP1 gamma antibody [MAC385] - ab10817 from Abcam

Prices	$359.00
Sizes	1 ml
Host	Rat
Clonality	Monoclonal
Clone	MAC385

Anti-HP1 gamma antibody - ChIP Grade - ab10480 from Abcam

Prices	$398.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ab10480 staining HP1 gamma in Mouse 14.5dpc tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 1 hour at room temperature; antigen retrieval was by heat mediation in Tris buffer pH 9. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS in TBS) for 16 hours. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview

Anti-CBX3 / HP1 Gamma Antibody - LS-C24210 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CBX3 / HP1 Gamma Antibody - LS-C24209 from LifeSpan Bioscience

Prices	$695.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal

Anti-CBX3 / HP1 Gamma Antibody (aa17-173) - LS-C24212 from LifeSpan Bioscience

Prices	$575.00
Sizes	200 µg
Host	Mouse
Clonality	Monoclonal

Anti-CBX3 / HP1 Gamma Antibody (phospho-Ser83) - LS-C67848 from LifeSpan Bioscience

Prices	$425.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CBX3 / HP1 Gamma Antibody (C-Terminus) - LS-C78016 from LifeSpan Bioscience

Prices	$545.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CBX3 / HP1 Gamma Antibody (aa1-50) - LS-C287815 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CBX3 / HP1 Gamma Antibody (C-Terminus) - LS-C292266 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CBX3 / HP1 Gamma Antibody (aa1-183, Biotin) - LS-C298397 from LifeSpan Bioscience

Prices	$435.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CBX3 / HP1 Gamma Antibody (aa1-183, Biotin) - LS-C298398 from LifeSpan Bioscience

Prices	$425.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CBX3 / HP1 Gamma Antibody (aa61-110) - LS-C30170 from LifeSpan Bioscience

Host	Rabbit
Clonality	Polyclonal

Anti-CBX3 / HP1 Gamma Antibody (aa71-120) - LS-C31773 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

CBX3 antibody LS-C31773 was used in IHC to stain formalin-fixed, paraffin-embedded human lung.

Anti-CBX3 / HP1 Gamma Antibody (aa1-183, FITC) - LS-C305082 from LifeSpan Bioscience

Prices	$455.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Search more