Western blot analysis of extracts from Ramos cells, serum starved for 12 hours, then treated with anti-IgM antibody or λ phosphatase, using Phospho-HS1 (Tyr397) (D12C1) XP ® Rabbit mAb (upper), or HS1 Antibody (Human Specific) #4503 (lower).
Confocal immunofluorescent analysis of Ramos cells, untreated (left) or treated with IgM (12 μg/mL, 10 min, right) using Phospho-HS1 (Tyr397) (D12C1) XP ® Rabbit mAb (green). Blue pseudocolor= DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of Ramos cells, untreated (blue) or IgM-treated (green), using Phospho-HS1 (Tyr397) (D12C1) XP ® Rabbit mAb.