PKC α (H-7): sc-8393. Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic staining.
Western blot analysis of PKC α expression in NIH/3T3 (A,C) and Jurkat (B) whole cell lysates. Antibodies tested include PKC α (C-20): sc-208 (A), PKC (MC5): sc-80 (B) and PKC α (H-7): sc-8393 (C).
Western blot detection of FADD phosphorylation using A-431 cells. Blots were probed with FADD (H-181): sc-5559 (A) and p-FADD (Ser 194)-R: sc-12439-R (B,C). In B and C, the antibody was preincubated with cognate non-phosphorylated or phosphorylated peptide, respectively.
PKC α (H-7): sc-8393. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon tumor showing cytoplasmic staining.
PKC α (H-7): sc-8393. Western blot analysis of PKC α expression in non-transfected 293T: sc-117752 (A), human PKC α transfected 293T: sc-177742 (B) and HeLa (C) whole cell lysates.
PKC α (H-7): sc-8393. Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.
Western blot analysis of PKC α phosphorylation in untreated (A,D),Ser/Thr Phosphorylation Induction Cocktail (sc-362324) treated (B,E) and Ser/Thr Phosphorylation Induction Cocktail (sc-362324) and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-PKC α (Ser 657)-R: sc-12356-R (A,B,C) and PKC α (H-7): sc-8393 (D,E,F).
Western blot analysis of PKC α phosphorylation in untreated (A,D),Ser/Thr Phosphorylation Induction Cocktail (sc-362324) treated (B,E) and Ser/Thr Phosphorylation Induction Cocktail (sc-362324) and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-PKC α (pT638.35): sc-136018 (A,B,C) and PKC α (H-7): sc-8393 (D,E,F).
Western blot analysis of PKC α phosphorylation in untreated (A,D),Ser/Thr Phosphorylation Induction Cocktail (sc-362324) treated (B,E) and Ser/Thr Phosphorylation Induction Cocktail (sc-362324) and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-PKC α (A-11): sc-377565 (A,B,C) and PKC α (H-7): sc-8393 (D,E,F).