Western blot analysis of PKC θ expression in CTLL-2 (A), Jurkat (B,C) and MOLT-4 (D) whole cell lysates. Antibodies tested include PKC θ (C-18)-G: sc-212-G (A,B) and PKC θ (C-19): sc-1875 (C,D).
Western blot analysis of PKC θ phosphorylation in PMA treated (A,C) and PMA and lambda protein phosphatase treated (B,D) Jurkat whole cell lysates. Antibodies tested include p-PKC θ (Ser 676): sc-33024 (A,B) and PKC θ (C-18): sc-212 (C,D).
Western blot analysis of PKC θ phosphorylation in PMA treated (A,C) and PMA and lambda protein phosphatase treated (B,D) Jurkat whole cell lysates. Antibodies tested include p-PKC θ (Thr 538): sc-33885 (A,B) and PKC θ (C-18): sc-212 (C,D).