PRKCQ

Gene Symbol PRKCQ
Entrez Gene 5588
Alt Symbol PRKCT, nPKC-theta
Species Human
Gene Type protein-coding
Description protein kinase C, theta
Other Description protein kinase C theta type
Swissprots Q64FY5 Q3MJF1 Q14DH6 Q9H508 B4DF52 Q9H549 Q04759
Accessions EAW86392 EAW86393 EAW86394 Q04759 AA234440 AI570478 AK293935 BAG57313 AK308099 AK312411 AU099414 AY702977 AAU29340 BC101465 AAI01466 BC113359 AAI13360 BC143222 BF592799 BX647657 HQ258485 ADR83239 L01087 AAA75571 L07032 AAA60101 R40214 XM_005252496 XP_005252553 XM_005252497 XP_005252554 XM_006717465 XP_006717528 XM_011519547 XP_011517849 XM_011519548 XP_011517850 NM_001242413 NP_001229342 NM_001282644 NP_001269573 NM_001282645 NP_001269574 NM_006257 NP_006248
Function Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non- redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates to the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non- canonical NF-kappa-B (NFKB2) pathway. In the signaling p
Subcellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.
Tissue Specificity Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets. {ECO:0000269|PubMed:7686153}.
Top Pathways Tight junction, T cell receptor signaling pathway, Adipocytokine signaling pathway, Vascular smooth muscle contraction, Measles

Phospho-PKCθ (Thr538) Antibody - 9377 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells, untreated (lane 1), TPA-treated (.2μM for 20 minutes) (lanes 2 & 3), and CIP/λ phosphatase treated (lane 3), using Phospho-PKCθ (Thr538) Antibody #9377, PKCθ Antibody #2059, and β-actin Antibody #4967.

PKCθ (P632) Antibody - 12206 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat and TALL-104 cells using PKCθ (P632) Antibody.

PKCθ (E1I7Y) Rabbit mAb - 13643 from Cell Signaling Technology

Prices $250.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1I7Y
Western blot analysis of extracts from wild-type and PKCθ (-/-) mouse splenocytes using PKCθ (E1I7Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Extracts from wild-type and PKCθ (-/-) mouse splenocytes were kindly provided by Dr. Morgan Huse (Memorial Sloan-Kettering Cancer Center).

PKCθ Antibody - 2059 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

PKC θ (C-18) - sc-212 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of PKC θ expression in CTLL-2 (A), Jurkat (B,C) and MOLT-4 (D) whole cell lysates. Antibodies tested include PKC θ (C-18)-G: sc-212-G (A,B) and PKC θ (C-19): sc-1875 (C,D).

PKC θ (C-19) - sc-1875 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PKC θ (C-19): sc-1875. Western blot analysis of PKC θ expression in MOLT-4 whole cell lysate.

PKC θ (E-7) - sc-1680 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKC θ (E-7): sc-1680. Western blot analysis of PKC θ expression in rat skeletal muscle (A) and rat thymus (B) tissue extracts and CTLL-2 whole cell lysate (C).

PKC θ (H-50) - sc-367101 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

PKC θ (1C2) - sc-81534 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Western blot analysis of PKC θ phosphorylation in untreated (A,C), PMA treated (B,E) and PMA and lambda protein phosphatase treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-PKC θ (A-11): sc-271922 (A,B,C) and PKC θ (1C2): sc-81534 (D,E,F).

p-PKC θ (A-11) - sc-271922 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of PKC θ phosphorylation in untreated (A,D), PMA treated (B,E) and PMA and lambda protein phosphatase treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-PKC θ (A-11): sc-271922 (A,B,C) and PKC θ (1C2): sc-81534 (D,E,F).

p-PKC θ (A-4) - sc-271920 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of PKC θ phosphorylation in untreated (A,D), PMA treated (B,E) and PMA and lambda protein phosphatase treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-PKC θ (A-4): sc-271920 (A,B,C) and PKC θ (1C2): sc-81534 (D,E,F).

p-PKC θ (Ser 676) - sc-33024 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of PKC θ phosphorylation in PMA treated (A,C) and PMA and lambda protein phosphatase treated (B,D) Jurkat whole cell lysates. Antibodies tested include p-PKC θ (Ser 676): sc-33024 (A,B) and PKC θ (C-18): sc-212 (C,D).

p-PKC θ (Ser 695) - sc-33025 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-PKC θ (Thr 538) - sc-33885 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of PKC θ phosphorylation in PMA treated (A,C) and PMA and lambda protein phosphatase treated (B,D) Jurkat whole cell lysates. Antibodies tested include p-PKC θ (Thr 538): sc-33885 (A,B) and PKC θ (C-18): sc-212 (C,D).

p-PKC θ (Thr 703) - sc-33026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-PKC θ (Ser 695) - sc-101780 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-PKC θ (Ser 695): sc-101780. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic staining.

p-PKC θ (Ser 676) - sc-101779 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-PKC θ (Ser 676): sc-101779. Western blot analysis of phosphorylated PKC θ expression in untreated (A) and PMA-treated (B) Jurkat whole cell lysates.

p-PKC θ (pT538.19) - sc-136017 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p-PKC θ (pT538.19): sc-136017. Western blot analysis of PKC θ expression in CD3-treated Jurkat whole cell lysates either untreated (A) or treated (B) with lambda phosphatase.

Anti-PKC theta (phospho S676) antibody - ab47774 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
This image shows human breast carcinoma stained with ab47774 at 1/50 dilution. Right hand image shows tissue treated with the immunogenic phosphopeptide, whereas the left hand image is untreated.

Anti-PKC theta (phospho S676) antibody - ab131479 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC theta (phospho S676) antibody (ab131479) at 1/500 dilutionLane 1 : PC12 cell extractLane 2 : PC12 cell extract treated with PMA

Anti-PKC theta (phospho S676) antibody - ab47384 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC theta (phospho S676) antibody (ab47384)Lane 1 : JK cell lysateLane 2 : Lysate of JK cells treated with PMA (200nM, 30min)

Anti-PKC theta (phospho S695) antibody - ab75658 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab75658 at 1/50 dilution staining PKC theta in human lung carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.

Anti-PKC theta (phospho T538) antibody - ab63365 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC theta (phospho T538) antibody (ab63365) at 1/500 dilutionLane 1 : Jurkat cell extractsLane 2 : Jurkat cell extracts with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-PKC theta (phospho T538) antibody [F4H4L1] - ab203565 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Monoclonal
Clone F4H4L1
All lanes : Anti-PKC theta (phospho T538) antibody [F4H4L1] (ab203565) at 3 µg/mlLane 1 : Jurkat lysates stimulated with 100 ng/mL PMA for 1 hourLane 2 : Jurkat lysates stimulated with 100 ng/mL PMA for 1 hour with Immunogen phosphopeptideLane 3 : Jurkat lysates stimulated with 100 ng/mL PMA for 1 hour with Non-phosphopeptide

Anti-PKC theta antibody - ab131503 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PKC theta antibody (ab131503) at 1/500 dilution + PC12 cell extract
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