Western blot detection of PKC a phosphorylation using HeLa nuclear extracts treated with PMA. Blots were probed with PKC a (H-7): sc-8393 (A) and PKC a (Ser 657)-R: sc-12356-R (B,C). Antibody was preincubated with cognate non-phosphorylated (B) or phosphorylated (C) peptide.
Western blot analysis of l phosphatase-treated (A,C) and untreated (B,D) active human recombinant PKC α. Antibodies tested include PKC (MC5): sc-80 (A,B) and p-PKC α (Ser 657): sc-12356 (C,D).
p-PKC α (Ser 657): sc-12356. Immunofluorescence staining of methanol-fixed PMA-treated HeLa cells showing cytoplasmic localization.
p-PKC α (Ser 657): sc-12356. Western blot analysis of PKC α phosphorylation in NIH/3T3 whole cell lysate.
Western blot analysis of PKC α phosphorylation in untreated (A,D),Ser/Thr Phosphorylation Induction Cocktail (sc-362324) treated (B,E) and Ser/Thr Phosphorylation Induction Cocktail (sc-362324) and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-PKC α (Ser 657)-R: sc-12356-R (A,B,C) and PKC α (H-7): sc-8393 (D,E,F).