Western blot analysis of PKC θ phosphorylation in PMA treated (A,C) and PMA and lambda protein phosphatase treated (B,D) Jurkat whole cell lysates. Antibodies tested include p-PKC θ (Thr 538): sc-33885 (A,B) and PKC θ (C-18): sc-212 (C,D).
Western blot analysis of PKC θ phosphorylation in untreated (A,D), PMA treated (B,E) and PMA and lambda protein phosphatase treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-PKC θ (Thr 538): sc-33885 (A,B,C) and PKC θ (1C2): sc-81534 (D,E,F).
Western blot analysis of PKC θ phosphorylation in untreated (A,D), PMA treated (B,E) and PMA and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-PKC θ (Thr 538): sc-33885 (A,B,C) and PKC θ (1C2): sc-81534 (D,E,F).