FOLR2 in Human Neutrophils. FOLR2 was detected in immersion fixed human neutrophils using Human FOLR2 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm.
Detection of Human FOLR2 by Western Blot. Western blot shows lysates of human placental tissue. PVDF membrane was probed with 1 ug/ml of Human FOLR2 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for FOLR2 at approximately 38 kD (as indicated). This experiment was conducted under reducing conditions.