Anti-bTrCP2 Antibody - Western Blot. Western blot of affinity purified anti-bTrCP2 antibody shows detection of mouse and human bTrCP2 (arrowhead) in NIH3T3 (lane 1) and 293 (lane 2) whole cell lysates, respectively. The band appears as a 58 kD protein, although a 62.1 kD band is predicted. The identity of faint higher molecular weight bands is not known. The primary antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4C. Detection occurred using HRP conjugated Goat-anti-Rabbit IgG (LS-C60865) diluted 1:20000 in blocking buffer (p/n MB-070) for 1 h at 4C.