Immunofluorescent of U251 cells, using FEN1 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Western blot of FEN1 (arrow) using rabbit polyclonal FEN1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FEN1 gene.
Western blot of anti-FEN1 Antibody in HepG2 cell line lysates (35 ug/lane). FEN1(arrow) was detected using the purified antibody.