Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a construct expressing full-length mouse HtrA2/Omi (mHtrA2/Omi; +), using HtrA2/Omi (D20A5) Rabbit mAb.
Western blot analysis of extracts from HeLa cells, untreated (upper) or treated with Staurosporine #9953 (1 μM, 3 hr; lower), using HtrA2/Omi (D20A5) Rabbit mAb #9745. Cells were fractionated into whole cell lysate (WCL), cytoplasm (Cyto), membrane (Mem), and cytoskeletal/nucleus (Nuc) using Cell Fractionation Kit #9038. Membrane fraction includes mitochondria.