![Localization of endogenous human Fis1 in HeLa cells. Method: Double staining with mtHSP70, mAb (JG1) [left] and using anti-Fis1, pAb [right] on methanol fixed cells reveals mitochondrial localization.](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_2/23396_210896_1528345.jpg)
Localization of endogenous human Fis1 in HeLa cells. Method: Double staining with mtHSP70, mAb (JG1) [left] and using anti-Fis1, pAb [right] on methanol fixed cells reveals mitochondrial localization.
Immunocytochemical staining and detection of endogenous Fis1 (red) (right) and endogenous mitochondrial Hsp70 (green) (left) in methanol fixed HeLa cells using anti-Fis1, pAb .Picture courtesy of P. Parone, University of Geneva.
Detection of endogenous Fis1 by Western blot (1:1000) using anti-Fis1, pAb . .Method: 10 ug mitochondrial proteins from mouse brain (A) and kidney (B); 10 ug cytosolic proteins from mouse liver (C).