IHC of paraffin-embedded NCIN87 xenograft using FIS1 antibody at 1:100 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa using TTC11 antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
TTC11 antibody detects FIS1 protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 15 % SDS-PAGE. TTC11 antibody dilution:1:1000
Sample (30 ug of whole cell lysate). A: Raji. 15% SDS PAGE. FIS1 antibody diluted at 1:10000.
Sample (50 ug of whole cell lysate). A: mouse brain. 15% SDS PAGE. FIS1 antibody diluted at 1:5000.