Western blot analysis of Raf-1 phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse Raf-1 transfected: sc-122942 (B,E) and lambda protein phosphatase (sc-200312A) treated mouse Raf-1 transfected: sc-122942 (C,F) 293T whole cell lysates. Antibodies tested include p-Raf-1 (H-8): sc-271928 (A,B,C) and Raf-1 (C-20): sc-227 (D,E,F).
Western blot analysis of Raf-1 phosphorylation in non-transfected: sc-110760 (A,D), untreated human Raf-1 transfected: sc-158911 (B,E) and lambda protein phosphatase (sc-200312A) treated human Raf-1 transfected: sc-158911 (C,F) 293 whole cell lysates. Antibodies tested include p-Raf-1 (H-8): sc-271928 (A,B,C) and Raf-1 (540): sc-52827 (D,E,F).
p-Raf-1 (H-8): sc-271928. Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic and nuclear staining of urothelial cells.