RAF1

Gene Symbol RAF1
Entrez Gene 5894
Alt Symbol CMD1NN, CRAF, NS5, Raf-1, c-Raf
Species Human
Gene Type protein-coding
Description Raf-1 proto-oncogene, serine/threonine kinase
Other Description C-Raf proto-oncogene, serine/threonine kinase|Oncogene RAF1|RAF proto-oncogene serine/threonine-protein kinase|proto-oncogene c-RAF|raf proto-oncogene serine/threonine protein kinase|v-raf-1 murine leukemia viral oncogene homolog 1
Swissprots Q9UC20 B0LPH8 P04049 Q15278 B2R5N3
Accessions AAA60247 AAP03432 ABY87557 AGC09606 CAD86886 CBX55729 CDL93489 EAW64134 EAW64135 P04049 AK226028 AK303561 BAG64584 AK303920 BAG64848 AK312248 BAG35180 BC018119 AAH18119 BQ221862 BU683389 BX648063 DC342671 X03484 CAA27204 XM_005265355 XP_005265412 XM_005265357 XP_005265414 XM_005265358 XP_005265415 XM_005265359 XP_005265416 XM_005265360 XP_005265417 XM_011533974 XP_011532276 XM_011533975 XP_011532277 NM_002880 NP_002871
Function Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2- antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in mot
Subcellular Location Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Note=Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid- induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.
Tissue Specificity In skeletal muscle, isoform 1 is more abundant than isoform 2. {ECO:0000269|PubMed:1886707}.
Top Pathways VEGF signaling pathway, Focal adhesion, Gap junction, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway

Phospho-c-Raf (Ser338) (56A6) Rabbit mAb - 9427 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 56A6
Western blot analysis of extracts from NIH3T3, HeLa and COS cells, untreated or treated with TPA, using Phospho-c-Raf (Ser338) (56A6) Rabbit mAb.

Phospho-c-Raf (Ser296) Antibody - 9423 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 and NIH/3T3 cells, untreated or treated with TPA + FBS, using Phospho-c-Raf (Ser296) Antibody.

Phospho-c-Raf (Ser289/296/301) Antibody - 9431 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from untreated or TPA-treated 293 and NIH/3T3 cells, using Phospho-c-Raf (Ser289/296/301) Antibody.

Phospho-c-Raf (Ser259) Antibody - 9421 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Site specificity of Phospho-c-Raf (Ser259) Antibody: Western blot analysis of recombinant Myc-tagged c-Raf protein, wild-type (lanes 1 and 3) and S259A mutant (lanes 2 and 4), using Phospho-Raf (Ser259) Antibody or a Myc antibody. (Provided by Dr. Guri Tzivion, Massachusetts General Hospital.)

c-Raf (D5X6R) Mouse mAb - 12552 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone D5X6R
Western blot analysis of extracts from various cell lines using c-Raf (D5X6R) Mouse mAb.

c-Raf Antibody - 9422 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, C2C12 or PC12 cells, untreated or TPA-treated (200 nM for 30 minutes), using c-Raf Antibody #9422.

Phospho-Raf (Ser338) Antibody - 9424 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-c-Raf (Ser289) Antibody - 9392 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Raf-1 (C-10) - sc-373722 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Raf-1 (C-10): sc-373722. Western blot analysis of Raf-1 expression in non-transfected: sc-117752 (A) and mouse Raf-1 transfected: sc-122942 (B) 293T whole cell lysates.

Raf-1 (H-8) - sc-376142 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Raf-1 (H-8): sc-376142. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Raf-1 (C-20) - sc-227 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Raf-1 (C-20): sc-227. Immunofluorescence staining of methanol-fixed 3611-RF cells showing cytoplasmic staining.

Raf-1 (E-10) - sc-7267 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Raf-1 (E-10): sc-7267. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and membrane staining.

Raf-1 (H-71) - sc-7198 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Raf-1 (H-71): sc-7198. Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic, membrane and nuclear staining of glandular cells.

Raf-1 (C-12) - sc-133 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of Raf-1 expression in KNRK (A), NIH/3T3 (B), 3611-RF (C,E) and HeLa (D) whole cell lysates. Antibodies tested include Raf-1 (C-12): sc-133 (A-D) and Raf-1 (C-20): sc-227 (E).

Raf-1 (540) - sc-52827 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Raf-1 (540): sc-52827. Western blot analysis of Raf-1 expression in non-transfected: sc-110760 (A) and human Raf-1 transfected: sc-158911 (B) 293 whole cell lysates.

p-Raf-1 (E-1) - sc-271929 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of Raf-1 phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse Raf-1 transfected: sc-122942 (B,E) and lambda protein phosphatase (sc-200312A) treated mouse Raf-1 transfected: sc-122942 (C,F) 293T whole cell lysates. Antibodies tested include p-Raf-1 (E-1): sc-271929 (A,B,C) and Raf-1 (C-20): sc-227 (D,E,F).

p-Raf-1 (H-8) - sc-271928 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of Raf-1 phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse Raf-1 transfected: sc-122942 (B,E) and lambda protein phosphatase (sc-200312A) treated mouse Raf-1 transfected: sc-122942 (C,F) 293T whole cell lysates. Antibodies tested include p-Raf-1 (H-8): sc-271928 (A,B,C) and Raf-1 (C-20): sc-227 (D,E,F).

p-Raf-1 (Ser 338) - sc-12358 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-Raf-1 (Ser 338)-R: sc-12358-R. Western blot analysis of Raf-1 phosphorylation in untreated (A) and UV-irradiated (B) HeLa cultures.

p-Raf-1 (Tyr 340/341) - sc-16806 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-Raf-1 (Tyr 340/341)-R: sc-16806-R. Western blot analysis of Raf-1 phosphorylation in non-transfected: sc-117752 (A) and human Raf-1 transfected: sc-158911 (B) 293T whole cell lysates.

p-Raf-1 (Ser 259) - sc-101791 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of phosphorylated Raf-1 expression in MDA-MB-435 (A,B) and K-562 (C) whole cell lysates. Antibodies tested include p-Raf-1 (Ser 259): sc-101791 preincubated with cognate phosphorylated peptide (A) and p-Raf-1 (Ser 259): sc-101791 (B,C).

p-Raf-1 (Ser 301) - sc-135707 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
p-Raf-1 (Ser 301): sc-135707. Western blot analysis of Raf-1 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) UV-treated Jurkat whole cell lysates.

p-Raf-1 (6B4) - sc-81513 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Western blot analysis of Raf-1 phosphorylation in non-transfected: sc-117752 (A, D), untreated mouse Raf-1 transfected: sc-122942 (B, E) and lambda protein phosphatase treated mouse Raf-1 transfected: sc-122942 (C, F) 293T whole cell lysates. Antibodies tested include p-Raf-1 (6B4): sc-81513 (A, B, C) and Raf-1 (540): sc-52827 (D, E, F).

Anti-Raf1 (phospho S259) antibody - ab192431 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Raf1 (phospho S259) antibody (ab192431) at 1/500 dilutionLane 1 : K562 whole cell lysates treated with the antigen-specific peptideLane 2 : K562 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-Raf1 (phospho S259) antibody - ab30621 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Ab30621, at a dilution of 1/50, staining Raf1 in paraffin embedded breast carcinoma tissue section by Immunohistochemistry.

Anti-Raf1 (phospho S259) antibody [EPR3433(2)] - ab173539 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3433(2)
All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilutionLane 1 : 293T cell lysatesLane 2 : 293T cell lysates treated with Okadaic Acid + Calyculin ALysates/proteins at 10 µg per lane.
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