Western blot analysis of phosphorylated Raf-1 expression in MDA-MB-435 (A,B) and K-562 (C) whole cell lysates. Antibodies tested include p-Raf-1 (Ser 259): sc-101791 preincubated with cognate phosphorylated peptide (A) and p-Raf-1 (Ser 259): sc-101791 (B,C).
p-Raf-1 (Ser 259): sc-101791. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.
Western blot analysis of Raf-1 phosphorylation in non-transfected: sc-117752 (A, D), untreated mouse Raf-1 transfected: sc-122942 (B, E) and lambda protein phosphatase treated mouse Raf-1 transfected: sc-122942 (C, F) 293T whole cell lysates. Antibodies tested include p-Raf-1 (Ser 259): sc-101791 (A, B, C) and Raf-1 (540): sc-52827 (D, E, F).
Western blot analysis of Raf-1 phosphorylation in non-transfected: sc-110760 (A,D), untreated human Raf-1 transfected: sc-158911 (B,E) and lambda protein phosphatase (sc-200312A) treated human Raf-1 transfected: sc-158911 (C,F) 293 whole cell lysates. Antibodies tested include p-Raf-1 (Ser 259): sc-101791 (A,B,C) and Raf-1 (540): sc-52827 (D,E,F).