Western blot analysis of inactive (A,C,E) and active (B,D,F) human recombinant SGK fusion protein. Antibodies tested include SGK (C-20): sc-15885 (A,B), p-SGK (Thr 256): sc-16744 (C,D) and p-SGK (Thr 256)-R: sc-16744-R (E,F).
p-SGK (Thr 256)-R: sc-16744-R. Western blot analysis of SGK phosphorylation in PC-12 whole cell lysate.
Western blot analysis of SGK phosphorylation in untreated (A,C) and lambda protein phosphatase treated (B,D) HeLa whole cell lysates. Antibodies tested include p-SGK (Thr 256)-R: sc-16744-R (A,B) and SGK (C-20): sc-15885 (C,D).
Western blot analysis of SGK phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) Hep G2 whole cell lysates. Antibodies tested include p-SGK (Thr 256)-R: sc-16744-R (A,B) and SGK (C-20): sc-15885 (C,D).