Fluorescent confocal image of HeLa cells stained with IFITM3 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with Fragilis (IFITM3) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi.
Western blot of lysate from HT1080 cell line,using IFITM3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
IFITM3 Antibody western blot of HeLa,293,A549,NCI-H460,HepG2,Sk-Br-3,A375 cell line lysates (35 ug/lane). The IFITM3 antibody detected the IFITM3 protein (arrow).