ZAP-70 (1E7.2) PE: sc-32760 PE. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-2866.
ZAP-70 (1E7.2): sc-32760. Western blot analysis of ZAP-70 expression in CCRF-CEM (A), BYDP (B), MOLT-4 (C) and Jurkat (D) whole cell lysates.
ZAP-70 (1E7.2): sc-32760. Western blot analysis of ZAP-70 expression in heterozygous (A) and wild type ZAP-70 expressing mouse thymocytes (B). Kindly provided by Dr. Arthur Weiss at the University of California at San Francisco, Howard Hughes Medical Institute.
ZAP-70 (1E7.2): sc-32760. Western blot analysis of ZAP-70 expression in non-transfected 293T: sc-117752 (A), truncated human ZAP-70 transfected 293T: sc-114635 (B) and Jurkat (C) whole cell lysates.
ZAP-70 (1E7.2): sc-32760. Western blot analysis of ZAP-70 expression in non-transfected 293T: sc-117752 (A), human ZAP-70 transfected 293T: sc-116483 (B) and CCRF-CEM (C) whole cell lysates.
ZAP-70 (1E7.2): sc-32760. Western blot analysis of ZAP-70 expression in non-transfected 293T: sc-117752 (A), mouse ZAP-70 transfected 293T: sc-124695 (B) and Jurkat (C) whole cell lysates.
Western blot analysis of ZAP-70 phosphorylation in untreated (A,D), pervanadate treated (B,E) and pervanadate and lambda protein phosphatase treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-ZAP-70 (Tyr 493): sc-33526 (A,B,C) and ZAP-70 (1E7.2): sc-32760 (D,E,F).
Western blot analysis of ZAP-70 phosphorylation in untreated (A,D), pervanadate treated (B,E) and pervanadate and lambda protein phosphatase treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-ZAP-70 (Tyr 493): sc-101823 (A,B,C) and ZAP-70 (1E7.2): sc-32760 (D,E,F).