ZAP70

Gene Symbol ZAP70
Entrez Gene 7535
Alt Symbol SRK, STCD, STD, TZK, ZAP-70
Species Human
Gene Type protein-coding
Description zeta-chain (TCR) associated protein kinase 70kDa
Other Description 70 kDa zeta-associated protein|70 kDa zeta-chain associated protein|syk-related tyrosine kinase|tyrosine-protein kinase ZAP-70|zeta-chain associated protein kinase, 70kD
Swissprots Q6PIA4 A6NFP4 Q9UBS6 P43403 Q8IXD6
Accessions AAX93187 EAX01918 EAX01919 P43403 AB083211 BAC43747 AK303341 BAG64404 AK310628 AK310637 AL832287 BC039039 AAH39039 BC053878 AAH53878 L05148 XM_005264015 XP_005264072 XM_006712728 XP_006712791 XM_011511783 XP_011510085 XR_923018 XR_923019 XR_923020 NM_001079 NP_001070 NM_207519 NP_997402
Function Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, ad
Subcellular Location Cytoplasm {ECO:0000269|PubMed:9813084}. Cell membrane {ECO:0000269|PubMed:9813084}; Peripheral membrane protein {ECO:0000269|PubMed:9813084}. Note=In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity). {ECO:0000250}.
Tissue Specificity Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells. {ECO:0000269|PubMed:1423621, ECO:0000269|PubMed:16467082, ECO:0000269|PubMed:9378960}.
Top Pathways Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, Primary immunodeficiency

Zap-70 (D1C10E) XP ® Rabbit mAb (PE Conjugate) - 14424 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D1C10E
Flow cytometric analysis of Ramos cells (blue) and Jurkat cells (green) using Zap-70 (D1C10E) XP ® Rabbit (PE Conjugate).

Zap-70 (D1C10E) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 9473 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D1C10E
Flow cytometric analysis of Ramos (blue) and Jurkat (green) cells using Zap-70 (D1C10E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate).

Zap-70 (136F12) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 2707 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 136F12
Flow cytometric analysis of Ramos B (blue) and Jurkat T (green) cells using Zap-70 (136F12) Rabbit mAb (Alexa Fluor ® 647 Conjugate).

Phospho-Zap-70 (Tyr493)/Syk (Tyr526) Antibody - 2704 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Ramos cells untreated or treated with 10 µg/ml IgM for 2 minutes with or without calf intestinal phosphatase (CIP), using Phospho-Zap-70 (Tyr493)/Syk (Tyr526) Antibody (upper) or Syk Antibody #12358 (lower).

Zap-70 (D1C10E) XP ® Rabbit mAb - 3165 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (20 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1C10E
Western blot analysis of extracts from Jurkat cells using Zap-70 (D1C10E) XP ® Rabbit mAb.

Zap-70 (99F2) Rabbit mAb - 2705 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 99F2
Western blot analysis of extracts from Jurkat cells, starved for 16 hours and either untreated or H2O2-treated (2 mM for 3 minutes), using Zap-70 (99F2) Rabbit mAb.

Zap-70 (L1E5) Mouse mAb - 2709 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone L1E5
Western blot analysis of extracts from Jurkat and Ramos cells, as well as 293 cells overexpressing Syk, using Zap70 (L1E5) Mouse mAb. Results show that this antibody does not cross-react with any B cell proteins (lane 2) or Syk (lane 3).

Zap-70 Antibody - 2702 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

ZAP-70 (1E7.2) - sc-32760 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ZAP-70 (1E7.2) PE: sc-32760 PE. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-2866.

ZAP-70 (A-1) - sc-365490 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ZAP-70 (A-1): sc-365490. Western blot analysis of ZAP-70 expression in Jurkat whole cell lysate.

ZAP-70 (A-8) - sc-374347 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ZAP-70 (A-8): sc-374347. Western blot analysis of ZAP-70 expression in Jurkat whole cell lysate.

ZAP-70 (G-14) - sc-30674 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ZAP-70 (G-14): sc-30674. Western blot analysis of ZAP-70 expression in Jurkat (A), Ramos (B), CCRF-CEM (C) and ALL-SIL (D) whole cell lysates.

ZAP-70 (G-4) - sc-17760 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ZAP-70 (G-4): sc-17760. Western blot analysis of ZAP-70 expression in Jurkat (A) and MOLT-4 (B) whole cell lysates.

ZAP-70 (LR) - sc-574 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ZAP-70 (LR): sc-574. Western blot analysis of ZAP-70 expression in CCRF-CEM (A) and BYDP (B) whole cell lysates.

ZAP-70 (3D2A4) - sc-53899 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ZAP-70 (3D2A4): sc-53899. Western blot analysis of ZAP-70 expression in Jurkat whole cell lysate under reducing (A) and non-reducing (B) conditions.

ZAP-70 (M-20) - sc-1526 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ZAP-70 (M-20): sc-1526. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic and membrane staining of cells in non-germinal centers.

ZAP-70 (N-15) - sc-30673 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ZAP-70 (N-15): sc-30673. Western blot analysis of ZAP-70 expression in Jurkat whole cell lysate.

Zap-70 (7) - sc-293085 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Zap-70 (7): sc-293085. Western blot analysis of Zap-70 expression in Jurkat whole cell lysate.

ZAP-70 (29) - sc-136305 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Zap-70 (29): sc-136305. Western blot analysis of Zap-70 expression in Jurkat whole cell lysate.

ZAP-70 (4H386) - sc-73279 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ZAP-70 (4H386): sc-73279. Western blot analysis of ZAP-70 expression in non-transfected 293T: sc-117752 (A), truncated human ZAP-70 transfected 293T: sc-114635 (B) and Jurkat (C) whole cell lysates.

ZAP-70 (24A) - sc-136126 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
ZAP-70 (24A): sc-136126. Western blot analysis of ZAP-70 expression in Jurkat whole cell lysate.

p-ZAP-70 (Tyr 292) - sc-12945 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-ZAP-70 (Tyr 292)-R: sc-12945-R. Western blot analysis of ZAP-70 phosphorylation in untreated (A), pervanadate treated (B) and pervanadate and lambda protein phosphatase treated (C) Jurkat whole cell lysates.

p-ZAP-70 (Tyr 493) - sc-33526 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-ZAP-70 (Tyr 493): sc-33526. Western blot analysis of ZAP-70 phosphoryation in Jurkat whole cell lysate.

p-ZAP-70 (Tyr 493) - sc-101823 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-ZAP-70 (Tyr 493): sc-101823. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.

p-ZAP-70 (Tyr 319) - sc-101822 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of phosphorylated ZAP-70 expression in Jurkat whole cell lysates. Antibodies tested include p-ZAP-70 (Tyr 319): sc-101822 preincubated with cognate phosphorylated peptide (A) and p-ZAP-70 (Tyr 319): sc-101822 (B).
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