GLS Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunohistochemical of paraffin-embedded H.kidney section using GLS Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Confocal immunofluorescent of GLS Antibody with HeLa cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Fluorescent image of HeLa cells stained with XAF1 GLS Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Fluorescent image of HeLa cells stained with XAF1 GLS Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot of lysates from 293, 293T, HeLa cell line , human brain and mouse brain tissue lysate (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of GLS Antibody in mouse liver tissue lysates (35 ug/lane). GLS (arrow) was detected using the purified antibody.
Western blot of lysates from human brain, mouse brain ad rat kidney tissue lysate (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of lysates from human brain and mouse brain tissue lysate (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of lysate from HeLa cell line, using GLS Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of lysates from 293T, HeLa cell line (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
GLS Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.