Western blot analysis of extracts from various cell lines using Integrin α4 (D2E1) XP ® Rabbit mAb.
Confocal immunofluorescent analysis of Jurkat (positive; left) and K-562 (negative; right) cells using Integrin α4 (D2E1) XP ® Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of Jurkat cells using Integrin α4 (D2E1) XP ® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.